Development of an accurate lateral flow immunoassay for PEDV detection in swine fecal samples with a filter pad design.

ABSTRACT: Porcine epidemic diarrhea virus (PEDV), as the main causative pathogen of viral diarrhea in pigs, has been reported to result in high morbidity and mortality in neonatal piglets and cause significant economic losses to the swine industry. Rapid diagnosis methods are essential for preventing outbreaks and transmission of this disease. In this study, a paper-based lateral flow immunoassay for the rapid diagnosis of PEDV in swine fecal samples was developed using stable color-rich latex beads as the label. Under optimal conditions, the newly developed latex bead-based lateral flow immunoassay (LBs-LFIA) attained a limit of detection (LOD) as low as 10^3.60 TCID₅₀/mL and no cross-reactivity with other related swine viruses. To solve swine feces impurity interference, by ad

SUBMITTER: Zou S 

PROVIDER: S-EPMC8572657 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature