Characterization and Application of a New β-Galactosidase Gal42 From Marine Bacterium Bacillus sp. BY02.

ABSTRACT: β-Galactosidase plays an important role in medicine and dairy industry. In this study, a new glycoside hydrolase family 42 (GH42) β-galactosidase-encoding gene, gal42, was cloned from a newly isolated marine bacterium Bacillus sp. BY02 and expressed in Escherichia coli. Structural characterization indicated that the encoding β-galactosidase, Gal42, is a homotrimer in solution, and homology modeling indicated that it retains the zinc binding sites of the Cys cluster. The reaction activity of Gal42 was significantly increased by Zn²⁺ (229.6%) and other divalent metal ions (Mn²⁺, Mg²⁺, and Co²⁺), while its activity was inhibited by EDTA (53.9%). Meanwhile, the thermo-stability of the Gal42 was also significantly enhanced by 5 an

SUBMITTER: Zhou Z 

PROVIDER: S-EPMC8573354 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature