Wnt ligands 3a and 5a regulate proliferation and migration in human fetal liver progenitor cells.

ABSTRACT:

Background

Since human fetal liver progenitor cells (hFLPC) can differentiate into multiple liver cell types in vitro and in vivo, hFLPC may be a suitable source for cell therapy and regeneration strategies. Imperative for effective clinical applications of hFLPC is the enhanced knowledge of growth factors that mediate and improve migration and proliferation. The canonical wingless/int-1 (Wnt) signal transduction pathway is known to play a key role in proliferation and migration of stem cells. So, we investigated a role for Wnt3a and Wnt5a ligands in regulating the proliferation and migration of hFLPC.

Methods

We used alamarBlue assay and transwell migration assay and examined proliferation and migration of hFLPC to Wnt3a and Wnt5a. In addition, the target gene

SUBMITTER: Liu Z 

PROVIDER: S-EPMC8573361 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature