Chloroplast import of an intermembrane space protein is facilitated by translocon components Toc75 and Tic236.

ABSTRACT: Chloroplasts are divided into six subcompartments: the outer membrane, intermembrane space, and inner membrane of the envelope, the stroma, the thylakoid membrane, and the thylakoid lumen. Compared with our knowledge of protein import into other subcompartments, extremely little is known about how proteins are imported into the intermembrane space of the envelope. Tic22 was one of the first proteins identified as localizing to the intermembrane space and the only one for which import has been analyzed in some detail. However, conflicting results have been obtained concerning whether the general translocon is used to import Tic22 into the intermembrane space. Taking advantage of available translocon component mutants, we reanalyzed import of Tic22. We reveal reduced in vitro import of Tic22

SUBMITTER: Chuang MR 

PROVIDER: S-EPMC8573378 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature