Molecular characterization of circulating avian metapneumovirus, subgroup B, in broiler chickens, Iran, 2016-2018.

ABSTRACT:

Background

Avian metapneumovirus (aMPV) infection has significant economic impacts on the poultry industry all around the world.

Aims

The aim of this study is molecular investigations of different types of aMPV in broiler farms in different provinces of Iran from 2016 to 2018.

Methods

Tracheal and oropharyngeal swabs were collected from two hundred broiler chickens with respiratory signs in ten provinces of Iran, including Kurdistan, West Azerbaijan, Semnan, Esfahan, Sistan and Baluchistan, Qazvin, Khuzestan, Fars, Gilan, and Khorasan Razavi from February 2016 to December 2018. After RNA extraction, the presence of aMPV was confirmed using N gene special primers. Then, subtype-specific primers were utilized to differentiate the specific subtype. All positive sa

SUBMITTER: Hosseini H 

PROVIDER: S-EPMC8573395 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature