ITPK1 is an InsP₆/ADP phosphotransferase that controls phosphate signaling in Arabidopsis.

ABSTRACT: In plants, phosphate (P_i) homeostasis is regulated by the interaction of PHR transcription factors with stand-alone SPX proteins, which act as sensors for inositol pyrophosphates. In this study, we combined different methods to obtain a comprehensive picture of how inositol (pyro)phosphate metabolism is regulated by P_i and dependent on the inositol phosphate kinase ITPK1. We found that inositol pyrophosphates are more responsive to P_i than lower inositol phosphates, a response conserved across kingdoms. Using the capillary electrophoresis electrospray ionization mass spectrometry (CE-ESI-MS) we could separate different InsP₇ isomers in Arabidopsis and rice, and identify 4/6-InsP₇ and a PP-InsP₄ isomer hitherto not reported i

SUBMITTER: Riemer E 

PROVIDER: S-EPMC8573591 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature