Starvation induced autophagy promotes the progression of bladder cancer by LDHA mediated metabolic reprogramming.

ABSTRACT:

Background

Aberrant autophagy and preternatural elevated glycolysis are prevalent in bladder cancer (BLCA) and are both related to malignant progression. However, the regulatory relationship between autophagy and glycolytic metabolism remains largely unknown. We imitated starvation conditions in the tumour microenvironment and found significantly increased levels of autophagy and aerobic glycolysis, which both regulated the progression of BLCA cells. We further explored the regulatory relationships and mechanisms between them.

Methods

We used immunoblotting, immunofluorescence and transmission electron microscopy to detect autophagy levels in BLCA cells under different treatments. Lactate and glucose concentration detection demonstrated changes in glycolysis. The expression

SUBMITTER: Li T 

PROVIDER: S-EPMC8573950 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature