CEBPG promotes acute myeloid leukemia progression by enhancing EIF4EBP1.

ABSTRACT:

Background

Acute myeloid leukemia (AML) is a myeloid neoplasm accounts for 7.6% of hematopoietic malignancies. AML is a complex disease, and understanding its pathophysiology is contributing to the improvement in the treatment and prognosis of AML. In this study, we assessed the expression profile and molecular functions of CCAAT enhancer binding protein gamma (CEBPG), a gene implicated in myeloid differentiation and AML progression.

Methods

shRNA mediated gene interference was used to down-regulate the expression of CEBPG in AML cell lines, and knockdown efficiency was detected by RT-qPCR and western blotting. The effect of knockdown on the growth of AML cell lines was evaluated by CCK-8. Western blotting was used to detect PARP cleavage, and flow cytometry were used to det

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC8574011 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature