N6-methyladenosine reader IMP2 stabilizes the ZFAS1/OLA1 axis and activates the Warburg effect: implication in colorectal cancer.

ABSTRACT:

Background

Accumulating evidence shows that N6-methyladenine (m⁶A) modulators contribute to the etiology and progression of colorectal cancer (CRC). However, the exact mechanisms of m⁶A reader involved in glycolytic metabolism remain vague. This article aimed to crosstalk the m⁶A reader with glycolytic metabolism and reveal a new mechanism for the progression of CRC.

Methods

The relationship between candidate lncRNA and m⁶A reader was analyzed by bioinformatics, ISH and IHC assays. In vivo and in vitro studies (including MTT, CFA, trans-well, apoptosis, western blot, qRT-PCR and xenograft mouse models) were utilized to explore the biological functions of these indicators. Lactate detection, ATP activity detection and ECAR assays we

SUBMITTER: Lu S 

PROVIDER: S-EPMC8574039 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature