Surface-induced dissociation of protein complexes on a cyclic ion mobility spectrometer.

ABSTRACT: We describe the implementation of a simple three-electrode surface-induced dissociation (SID) cell on a cyclic ion mobility spectrometer (cIMS) and demonstrate the utility of multipass mobility separations for resolving multiple conformations of protein complexes generated during collision-induced and surface-induced unfolding (CIU & SIU) experiments. In addition to CIU and SIU, SID of protein complexes is readily accomplished within the native instrument software and with no additional external power supplies by entering a single SID collision energy, a simplification in user experience compared to prior implementations. A set of cyclic homomeric protein complexes and a heterohexamer with known CID and SID behavior were analyzed to investigate mass and mobility resolution improvements, th

SUBMITTER: Snyder DT 

PROVIDER: S-EPMC8574189 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature