Quantitative Detection of Anti-SARS-CoV-2 Antibodies Using Indirect ELISA.

ABSTRACT:

Objective

Real-time reverse transcription-polymerase chain reaction is the gold standard for the diagnosis of COVID-19, but it is necessary to utilize other tests to determine the burden of the disease and the spread of the outbreak such as IgG-, IgM-, and IgA-based antibody detection using enzyme-linked immunosorbent assay (ELISA).

Materials and methods

We developed an indirect ELISA assay to quantitatively measure the amount of COVID-19 IgG, IgM, and IgA antibodies present in patient serum, dried blood, and plasma.

Results

The population cutoff values for positivity were determined by receiver operating characteristic curves to be 1.23 U/mL, 23.09 U/mL, and 6.36 U/mL for IgG, IgM, and IgA, respectively. After albumin subtraction, the specificity remained >98% and th

SUBMITTER: Luo S 

PROVIDER: S-EPMC8574490 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature