ARAMIS: From systematic errors of NGS long reads to accurate assemblies.

ABSTRACT: NGS long-reads sequencing technologies (or third generation) such as Pacific BioSciences (PacBio) have revolutionized the sequencing field over the last decade improving multiple genomic applications like de novo genome assemblies. However, their error rate, mostly involving insertions and deletions (indels), is currently an important concern that requires special attention to be solved. Multiple algorithms are available to fix these sequencing errors using short reads (such as Illumina), although they require long processing times and some errors may persist. Here, we present Accurate long-Reads Assembly correction Method for Indel errorS (ARAMIS), the first NGS long-reads indels correction pipeline that combines several correction software in just one step using accurate short reads. As

SUBMITTER: Sacristan-Horcajada E 

PROVIDER: S-EPMC8574707 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature