Endolysosomal ion channel MCOLN2 (Mucolipin-2) promotes prostate cancer progression via IL-1β/NF-κB pathway.

ABSTRACT:

Background

Prostate cancer (Pca) is the most common cancer type among males worldwide. Dysregulation of Ca²⁺ signaling plays important roles during Pca progression. However, there is lack of information about the role of endolysosomal Ca²⁺ -permeable channels in Pca progression.

Methods

The expression pattern of MCOLN2 was studied by immunohistochemistry and western blot. Cell viability assay, transwell assay and in vivo tumorigenesis were performed to evaluate the functional role of MCOLN2. Downstream targets of MCOLN2 were investigated by cytokine array, enzyme-linked immunosorbent assay, Ca²⁺ release experiments and luciferase reporter assays.

Results

We report that MCOLN2 expression is significantly elevated in Pca tissues, and ass

SUBMITTER: Yu H 

PROVIDER: S-EPMC8575885 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature