Multi-omics mapping of human papillomavirus integration sites illuminates novel cervical cancer target genes.

ABSTRACT:

Background

Integration of human papillomavirus (HPV) into the host genome is a dominant feature of invasive cervical cancer (ICC), yet the tumorigenicity of cis genomic changes at integration sites remains largely understudied.

Methods

Combining multi-omics data from The Cancer Genome Atlas with patient-matched long-read sequencing of HPV integration sites, we developed a strategy for using HPV integration events to identify and prioritise novel candidate ICC target genes (integration-detected genes (IDGs)). Four IDGs were then chosen for in vitro functional studies employing small interfering RNA-mediated knockdown in cell migration, proliferation and colony formation assays.

Results

PacBio data revealed 267 unique human-HPV breakpoints comprising 87 total integratio

SUBMITTER: Iden M 

PROVIDER: S-EPMC8575955 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature