Molecular epidemiology and phylogenetic analysis of bovine picobirnaviruses causing calf diarrhea, in Iran.

ABSTRACT: Picobirnavirus (PBV) is an enteropathogen virus causing diarrhea as an opportunistic virus in its vertebrate host. There is no information about human or animal PBVs in Iran. The aim of the present study was the investigation of the epidemiology of bovine PBV in the broad geographical area of Iran. Four hundred and eighty-five stool samples of up to 1 month old diarrheic calves were collected from 14 provinces and were tested with polyacrylamide gel electrophoresis (PAGE), and reverse transcription polymerase chain reaction (RT-PCR). Five samples were positive in PAGE assay (1.00%) and all of them were amplified using GI specific primers in RT-PCR. Phylogenetic analysis of one of the amplicons (strain Nazaktabar-14) revealed a low relationship to bovine PBV sequences and more identity to P

SUBMITTER: Nazaktabar A 

PROVIDER: S-EPMC8576153 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature