An accurate, rapid and simple loop-mediated isothermal amplification method for Explanatum explanatum detection in animals.

ABSTRACT: Paramphistomosis is an infectious disease caused by the liver flukes and it is associated with heavy loss of ruminant's population. Explanatum explanatum is a digenetic trematode commonly affecting domesticated ruminants. The available methods for pathogen detection are laborious and expensive and offer limited specificity; thus, considered not suitable for post mortem pathogen detection, surveillance and prevalence studies. New detection techniques offering simplicity, specificity and rapidity are absolutely needed. We have designed a loop-mediated isothermal amplification (LAMP) based polymerase chain reaction method, targeting a sequence of the explanatum species, using a primer pair from the internal transcribed spacer-2 region. The DNA from adult flukes belonging to e

SUBMITTER: Rashid M 

PROVIDER: S-EPMC8576162 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature