Profile of Podocyte Translatome During Development of Type 2 and Type 1 Diabetic Nephropathy Using Podocyte-Specific TRAP mRNA RNA-seq.

ABSTRACT: Podocyte injury is important in development of diabetic nephropathy (DN). Although several studies have reported single-cell-based RNA sequencing (RNA-seq) of podocytes in type 1 DN (T1DN), the podocyte translating mRNA profile in type 2 DN (T2DN) has not previously been compared with that of T1DN. We analyzed the podocyte translatome in T2DN in podocin-Cre; Rosa26^fsTRAP; eNOS^-/-; db/db mice and compared it with that of streptozotocin-induced T1DN in podocin-Cre; Rosa26^fsTRAP; eNOS^-/- mice using translating ribosome affinity purification (TRAP) and RNA-seq. More than 125 genes were highly enriched in the podocyte ribosome. More podocyte TRAP genes were differentially expressed in T2DN than in T1DN. TGF-β signaling pathway genes were upregu

SUBMITTER: Wang Y 

PROVIDER: S-EPMC8576501 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature