Towards Engineering an Orthogonal Protein Translation Initiation System.

ABSTRACT: In the last two decades, methods to incorporate non-canonical amino acids (ncAAs) into specific positions of a protein have advanced significantly; these methods have become general tools for engineering proteins. However, almost all these methods depend on the translation elongation process, and strategies leveraging the initiation process have rarely been reported. The incorporation of a ncAA specifically at the translation initiation site enables the installation of reactive groups for modification at the N-termini of proteins, which are attractive positions for introducing abiological groups with minimal structural perturbations. In this study, we attempted to engineer an orthogonal protein translation initiation system. Introduction of the identity elements of Escherichia coli

SUBMITTER: Lee BS 

PROVIDER: S-EPMC8576571 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature