Anticancer activity of ursolic acid on retinoblastoma cells determined by bioinformatics analysis and validation.

ABSTRACT:

Background

This article aims to explore whether ursolic acid (UA) inhibits the progression of retinoblastoma (Rb) by regulating stearoyl-CoA desaturase (SCD).

Methods

The Gene Expression Omnibus (GEO) database was used to filter the chip, then the GEO2R software was used to analyze the microarray data (GSE97508, GSE24673, and GSE110811). Gene set enrichment analysis (GSEA) was used to analyze the relationship between the expression level of SCD and the proliferation, migration, invasion, and inflammation in Rb patients. SO-RB50 and Y79 cell proliferation, migration, and invasion were assessed by the CCK-8 assay, the colony formation assay, the Transwell assay, and the wound scratch test. The protein expression levels of SCD were measured by western blot. The mRNA expression

SUBMITTER: Zhou D 

PROVIDER: S-EPMC8576664 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature