Reducing l-lactate release from hippocampal astrocytes by intracellular oxidation increases novelty induced activity in mice.

ABSTRACT: Astrocytes are in control of metabolic homeostasis in the brain and support and modulate neuronal function in various ways. Astrocyte-derived l-lactate (lactate) is thought to play a dual role as a metabolic and a signaling molecule in inter-cellular communication. The biological significance of lactate release from astrocytes is poorly understood, largely because the tools to manipulate lactate levels in vivo are limited. We therefore developed new viral vectors for astrocyte-specific expression of a mammalianized version of lactate oxidase (LOx) from Aerococcus viridans. LOx expression in astrocytes in vitro reduced their intracellular lactate levels as well as the release of lactate to the extracellular space. Selective expression of LOx in astrocytes of the dorsal hippocampus in mice r

SUBMITTER: Vaccari Cardoso B 

PROVIDER: S-EPMC8576740 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature