Sperm chromatin condensation as an in vivo fertility biomarker in bulls: a flow cytometry approach.

ABSTRACT:

Background

Genetic selection in cattle has been directed to increase milk production. This, coupled to the fact that the vast majority of bovine artificial inseminations (AI) are performed using cryopreserved sperm, have led to a reduction of fertility rates over the years. Thus, seeking sensitive and specific sperm biomarkers able to predict fertility rates is of vital importance to improve cattle reproductive efficiency. In humans, sperm chromatin condensation evaluated through chromomycin A3 (CMA3) has recently been purported to be a powerful biomarker for sperm functional status and male infertility. The objectives of the present study were: a) to set up a flow cytometry method for simultaneously evaluating chromatin condensation and sperm viability, and b) to test whether this

SUBMITTER: Llavanera M 

PROVIDER: S-EPMC8576882 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature