Cancer cell-derived exosomal circUSP7 induces CD8⁺ T cell dysfunction and anti-PD1 resistance by regulating the miR-934/SHP2 axis in NSCLC.

ABSTRACT:

Background

CD8⁺ T cells play a critical role in the innate antitumour immune response. Recently, CD8⁺ T cell dysfunction has been verified in various malignant cancers, including non-small cell lung cancer (NSCLC). However, the molecular biological mechanisms of CD8⁺ T cell dysfunction in human NSCLC are still unclear.

Methods

The expression of circular ubiquitin-specific protease-7 (circUSP7) in NSCLC tissues, exosomes, and cell lines was detected by quantitative real-time polymerase chain reaction (qRT-PCR). Exosomes were isolated from the culture medium of NSCLC cells and the plasma of NSCLC patients using an ultracentrifugation method and the ExoQuick Exosome Precipitation Solution kit. The exosomes were then characterized by transmission

SUBMITTER: Chen SW 

PROVIDER: S-EPMC8576933 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature