RPD3 and UME6 are involved in the activation of PDR5 transcription and pleiotropic drug resistance in ρ⁰ cells of Saccharomyces cerevisiae.

ABSTRACT:

Background

In Saccharomyces cerevisiae, the retrograde signalling pathway is activated in ρ^0/- cells, which lack mitochondrial DNA. Within this pathway, the activation of the transcription factor Pdr3 induces transcription of the ATP-binding cassette (ABC) transporter gene, PDR5, and causes pleiotropic drug resistance (PDR). Although a histone deacetylase, Rpd3, is also required for cycloheximide resistance in ρ^0/- cells, it is currently unknown whether Rpd3 and its DNA binding partners, Ume6 and Ash1, are involved in the activation of PDR5 transcription and PDR in ρ^0/- cells. This study investigated the roles of RPD3, UME6, and ASH1 in the activation of PDR5 transcription and PDR by retrograde signalling in ρ⁰ cells.

Results

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SUBMITTER: Yamada Y 

PROVIDER: S-EPMC8576940 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature