Phylogenetic analysis and in-depth characterization of functionally and structurally diverse CE5 cutinases.

ABSTRACT: Cutinases are esterases that release fatty acids from the apoplastic layer in plants. As they accept bulky and hydrophobic substrates, cutinases could be used in many applications, ranging from valorization of bark-rich side streams to plastic recycling. Advancement of these applications, however, requires deeper knowledge of cutinases' biodiversity and structure-function relationships. Here, we mined over 3000 members from carbohydrate esterase family 5 for putative cutinases and condensed it to 151 genes from known or putative lignocellulose-targeting organisms. The 151 genes were subjected to a phylogenetic analysis, which showed that cutinases with available crystal structures were phylogenetically closely related. We then selected nine phylogenic diverse cutinases for recombinant prod

SUBMITTER: Novy V 

PROVIDER: S-EPMC8577158 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature