Rescue of male infertility through correcting a genetic mutation causing meiotic arrest in spermatogonial stem cells.

ABSTRACT: Azoospermia patients who carry a monogenetic mutation that causes meiotic arrest may have their biological child through genetic correction in spermatogonial stem cells (SSCs). However, such therapy for infertility has not been experimentally investigated yet. In this study, a mouse model with an X-linked testis-expressed 11 (TEX11) mutation (Tex11^PM/Y) identified in azoospermia patients exhibited meiotic arrest due to aberrant chromosome segregation. Tex11^PM/Y SSCs could be isolated and expanded in vitro normally, and the mutation was corrected by clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated endonuclease 9 (Cas9), leading to the generation of repaired SSC lines. Whole-genome sequencing demonstrated that the mutation rate in rep

SUBMITTER: Wang YH 

PROVIDER: S-EPMC8577253 | biostudies-literature | 2021 Nov-Dec

REPOSITORIES: biostudies-literature