Direct RNA Sequencing Unfolds the Complex Transcriptome of Vibrio parahaemolyticus.

ABSTRACT: Conventional bacterial genome annotation provides information about coding sequences but ignores untranslated regions and operons. However, untranslated regions contain important regulatory elements as well as targets for many regulatory factors, such as small RNAs. Operon maps are also essential for functional gene analysis. In the last decade, considerable progress has been made in the study of bacterial transcriptomes through transcriptome sequencing (RNA-seq). Given the compact nature of bacterial genomes, many challenges still cannot be resolved through short reads generated using classical RNA-seq because of fragmentation and loss of the full-length information. Direct RNA sequencing is a technology that sequences the native RNA directly without information loss or bias. Here, we emp

SUBMITTER: Al Kadi M 

PROVIDER: S-EPMC8577284 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature