Cisd2 plays an essential role in corneal epithelial regeneration.

ABSTRACT:

Background

Age-related changes affecting the ocular surface cause vision loss in the elderly. Cisd2 deficiency drives premature aging in mice as well as resulting in various ocular surface abnormalities. Here we investigate the role of CISD2 in corneal health and disease.

Methods

We studied the molecular mechanism underlying the ocular phenotypes brought about by Cisd2 deficiency using both Cisd2 knockout (KO) mice and a human corneal epithelial cell (HCEC) cell line carrying a CRISPR-mediated CISD2KO background. We also develop a potential therapeutic strategy that targets the Ca²⁺ signaling pathway, which has been found to be dysregulated in the corneal epithelium of subjects with ocular surface disease in order to extend the mechanistic findings into a translat

SUBMITTER: Sun CC 

PROVIDER: S-EPMC8577409 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature