The Role of Ca^{2 +} in Maturation and Reprogramming of Bovine Oocytes: A System Study of Low-Calcium Model.

ABSTRACT: [Ca²⁺]_i is essential for mammalian oocyte maturation and early embryonic development, as those processes are Ca²⁺ dependent. In the present study, we investigated the effect of [Ca²⁺]_i on in vitro maturation and reprogramming of oocytes in a lower calcium model of oocyte at metaphase II (MII) stage, which was established by adding cell-permeant Ca²⁺ chelator BAPTA-AM to the maturation medium. Results showed that the extrusion of the first polar body (PB1) was delayed, and oocyte cytoplasmic maturation, including mitochondrial and endoplasmic reticulum distribution, was impaired in lower calcium model. The low-calcium-model oocytes presented a poor developmental phenotype of somatic cell nuclear transfer (SCNT) embryos

SUBMITTER: Meng L 

PROVIDER: S-EPMC8577575 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature