Protectin DX promotes the inflammatory resolution via activating COX-2/L-PGDS-PGD₂ and DP₁ receptor in acute respiratory distress syndrome.

ABSTRACT:

Purpose

Acute respiratory distress syndrome (ARDS) is characterized by uncontrollable inflammation. Cyclooxygenase-2(COX-2) and its metabolite prostaglandins are known to promote the inflammatory resolution of ARDS. Recently, a newly discovered endogenous lipid mediator, Protectin DX (PDX), was also shown to mediate the resolution of inflammation. However, the regulatory of PDX on the pro-resolving COX-2 in ARDS remains unknown.

Material and methods

PDX (5 μg/kg) was injected into rats intravenously 12 h after the lipopolysaccharide (LPS, 3 mg/kg) challenge. Primary rat lung fibroblasts were incubated with LPS (1 μg/ml) and/or PDX (100 nM). Lung pathological changes examined using H&E staining. Protein levels of COX-2, PGDS and PGES were evaluated using western blot. Inflamm

SUBMITTER: Hu X 

PROVIDER: S-EPMC8578004 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature