Generation of a Novel In Vitro Model to Study Endothelial Dysfunction from Atherothrombotic Specimens.

ABSTRACT:

Purpose

Endothelial dysfunction is central to the pathogenesis of acute coronary syndrome. The study of diseased endothelium is very challenging due to inherent difficulties in isolating endothelial cells from the coronary vascular bed. We sought to isolate and characterise coronary endothelial cells from patients undergoing thrombectomy for myocardial infarction to develop a patient-specific in vitro model of endothelial dysfunction.

Methods

In a prospective cohort study, 49 patients underwent percutaneous coronary intervention with thrombus aspiration. Specimens were cultured, and coronary endothelial outgrowth (CEO) cells were isolated. CEO cells, endothelial cells isolated from peripheral blood, explanted coronary arteries, and umbilical veins were phenotyped and assesse

SUBMITTER: Gallogly S 

PROVIDER: S-EPMC8578063 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature