Electron carriers involved in autotrophic and heterotrophic acetogenesis in the thermophilic bacterium Thermoanaerobacter kivui.

ABSTRACT: Thermoanaerobacter kivui is an acetogenic model organism that reduces CO₂ with electrons derived from H₂ or CO, or from organic substrates in the Wood-Ljugdahl pathway (WLP). For the calculation of ATP yields, it is necessary to know the electron carriers involved in coupling of the oxidative and reductive parts of metabolism. Analyses of key catabolic oxidoreductases in cell-free extract (CFE) or with purified enzymes revealed the physiological electron carriers involved. The glyceraldehyde-3-phosphate dehydrogenase (GA3P-DH) assayed in CFE was NAD⁺-specific, NADP⁺ was used with less than 4% and ferredoxin (Fd) was not used. The methylene-THF dehydrogenase was NADP⁺-specific, NAD⁺ or Fd were not used. A Nfn-type transhydrog

SUBMITTER: Katsyv A 

PROVIDER: S-EPMC8578170 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature