Active B-Type Natriuretic Peptide Measured by Mass Spectrometry and Response to Sacubitril/Valsartan.

ABSTRACT:

Background

B-type natriuretic peptide (BNP) immunoassays (BNP_ia) do not differentiate active and inactive forms. Inactive NT-proBNP is used to track heart failure (HF) during treatment with sacubitril/valsartan, which inhibits BNP degradation. Mass spectrometry (MS) may better assess effects of HF treatment on biologically active BNP1-32.

Methods and results

We developed a MS assay with immediate protease inhibition to quantify BNP1-32 over a linear range, using labeled recombinant BNP standard. In 4 healthy volunteers, BNP1-32 by MS (BNP_MS) increased from below the 5 pg/mL detection limit to 228 pg/mL after nesiritide. In patients with HF, BNP_MS was measured in parallel with BNP and NT-proBNP immunoassays before and during sacubitril/valsart

SUBMITTER: Dillon EM 

PROVIDER: S-EPMC8578199 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature