Expression of F-actin and β-tubulin genes in free mycelia and robust biofilms of the filamentous fungus Aspergillus niger.

ABSTRACT: The morphology and growth of the filamentous fungi are influenced by different factors as the culture conditions and the type of fermentative process. The production and secretion of metabolites by these organisms present a direct relationship with their morphology. The organization of the microtubules and actin in the cytoskeleton is determinant for both the fungal growth and morphology. In this context, this study aimed to analyze the expression of the β-tubulin, F-actin, and glucan synthase in the A. niger mycelia obtained from submerged fermentation and biofilm fermentation through qPCR, as well as the analysis of the nucleus distribution in the hypha. Herein, we showed that β-tubulin and the F-actin gene were more expressed in the biofilm condition, while the glucan synthase was in th

SUBMITTER: Guimaraes LHS 

PROVIDER: S-EPMC8578212 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature