A microfluidic platform enables comprehensive gene expression profiling of mouse retinal stem cells.

ABSTRACT: Loss of photoreceptors due to retinal degeneration is a major cause of untreatable visual impairment and blindness. Cell replacement therapy, using retinal stem cell (RSC)-derived photoreceptors, holds promise for reconstituting damaged cell populations in the retina. One major obstacle preventing translation to the clinic is the lack of validated markers or strategies to prospectively identify these rare cells in the retina and subsequently enrich them. Here, we introduce a microfluidic platform that combines nickel micromagnets, herringbone structures, and a design enabling varying flow velocities among three compartments to facilitate a highly efficient enrichment of RSCs. In addition, we developed an affinity enrichment strategy based on cell-surface markers that was utilized to isolat

SUBMITTER: Coles BLK 

PROVIDER: S-EPMC8578462 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature