Patch-seq of mouse DRG neurons reveals candidate genes for specific mechanosensory functions.

ABSTRACT: A variety of mechanosensory neurons are involved in touch, proprioception, and pain. Many molecular components of the mechanotransduction machinery subserving these sensory modalities remain to be discovered. Here, we combine recordings of mechanosensitive (MS) currents in mechanosensory neurons with single-cell RNA sequencing. Transcriptional profiles are mapped onto previously identified sensory neuron types to identify cell-type correlates between datasets. Correlation of current signatures with single-cell transcriptomes provides a one-to-one correspondence between mechanoelectric properties and transcriptomically defined neuronal populations. Moreover, a gene-expression differential comparison provides a set of candidate genes for mechanotransduction complexes. Piezo2 is expectedly fo

SUBMITTER: Parpaite T 

PROVIDER: S-EPMC8578708 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature