Immunological Study of Reconstructed Common Ancestral Sequence of Adenovirus Hexon Protein.

ABSTRACT: Aim: To reconstruct the ancestral sequence of human adenoviral hexon protein by combining sequence variations and structural information. And to provide a candidate hexon protein for developing new adenoviral vector capable of escaping the pre-existing immunity in healthy populations. Methods: The sequences of 74 adenovirus-type strains were used to predict the ancestral sequence of human adenovirus hexon protein using FastML and MEGA software. The three-dimensional structure model was built using homology modeling methods. The immunological features of ancestral loop 1 and loop 2 regions of sequences were tested using protein segments expressed in a prokaryotic expression system and polypeptides synthesized with human serum samples. Results: The tower region of the he

SUBMITTER: Wang Y 

PROVIDER: S-EPMC8578728 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature