The Alterations in Methylene Blue/Light-Treated Frozen Plasma Proteins Revealed by Proteomics.

ABSTRACT:

Introduction

The aim of this study was to investigate the modified proteins in methylene blue/light-treated frozen plasma (MB-FP) compared with fresh frozen plasma (FFP) in order to gain a better application of MB/light-treated plasma in clinic transfusion.

Methods

MB-FP and FFP were collected from Changchun central blood station, and a trichloroacetic acid/acetone precipitation method was used to remove albumin for the enrichment of lower abundance proteins. The plasma protein in MB-FP and FFP were separated using two-dimensional gel electrophoresis (2-DE) and the differentially expressed protein spots were analyzed using mass spectrometry. Finally, the differentially expressed proteins were tested using Western blot and enzyme-linked immunosorbent assay (ELISA).

Results

SUBMITTER: Wu T 

PROVIDER: S-EPMC8578803 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature