Bioluminescent Sensors for Ca⁺⁺ Flux Imaging and the Introduction of a New Intensity-Based Ca⁺⁺ Sensor.

ABSTRACT: Sensitive detection of biological events is a goal for the design and characterization of sensors that can be used in vitro and in vivo. One important second messenger is Ca⁺⁺ which has been a focus of using genetically encoded Ca⁺⁺ indicators (GECIs) within living cells or intact organisms in vivo. An ideal GECI would exhibit high signal intensity, excellent signal-to-noise ratio (SNR), rapid kinetics, a large dynamic range within relevant physiological conditions, and red-shifted emission. Most available GECIs are based on fluorescence, but bioluminescent GECIs have potential advantages in terms of avoiding tissue autofluorescence, phototoxicity, photobleaching, and spectral overlap, as well as enhancing SNR. Here, we summarize current progress

SUBMITTER: Yang J 

PROVIDER: S-EPMC8578923 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature