Dataset Information

Stomatal Responses to Light, CO₂, and Mesophyll Tissue in Vicia faba and Kalanchoe fedtschenkoi.

ABSTRACT: The responses of stomatal aperture to light intensity and CO₂ concentration were studied in both Vicia faba (C₃) and Kalanchoë fedtschenkoi (Crassulacean acid metabolism; CAM), in material sampled from both light and dark periods. Direct comparison was made between intact leaf segments, epidermises grafted onto exposed mesophyll, and isolated epidermal peels, including transplantations between species and between diel periods. We reported the stomatal opening in response to darkness in isolated CAM peels from the light period, but not from the dark. Furthermore, we showed that C₃ mesophyll has stimulated CAM stomata in transplanted peels to behave as C₃ in response to light and CO₂. By using peels and mesophyll from plants sampled in the dark and the light period, we provided clear evidence that CAM stomata behaved differently from C₃. This might be linked to stored metabolites/ions and signalling pathway components within the guard cells, and/or a mesophyll-derived signal. Overall, our results provided evidence for both the involvement of guard cell metabolism and mesophyll signals in stomatal responses in both C₃ and CAM species.

SUBMITTER: Santos MG

PROVIDER: S-EPMC8579043 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Stomatal Responses to Light, CO2, and Mesophyll Tissue in Vicia faba and Kalanchoë fedtschenkoi.

Santos Mauro G MG Davey Phillip A PA Hofmann Tanja A TA Borland Anne A Hartwell James J Lawson Tracy T

Frontiers in plant science 20211027

The responses of stomatal aperture to light intensity and CO2 concentration were studied in both Vicia faba (C3) and Kalanchoë fedtschenkoi (Crassulacean acid metabolism; CAM), in material sampled from both light and dark periods. Direct comparison was made between intact leaf segments, epidermises grafted onto exposed mesophyll, and isolated epidermal peels, including transplantations between species and between diel periods. We reported the stomatal opening ...[more]

PMID: 34777422

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Project description:BACKGROUND:Crassulacean acid metabolism (CAM), a specialized mode of photosynthesis, enables plant adaptation to water-limited environments and improves photosynthetic efficiency via an inorganic carbon-concentrating mechanism. Kalanchoë fedtschenkoi is an obligate CAM model featuring a relatively small genome and easy stable transformation. However, the molecular responses to light quality and intensity in CAM plants remain understudied. RESULTS:Here we present a genome-wide expression atlas of K. fedtschenkoi plants grown under 12 h/12 h photoperiod with different light quality (blue, red, far-red, white light) and intensity (0, 150, 440, and 1,000 ?mol m-2 s-1) based on RNA sequencing performed for mature leaf samples collected at dawn (2 h before the light period) and dusk (2 h before the dark period). An eFP web browser was created for easy access of the gene expression data. Based on the expression atlas, we constructed a light-responsive co-expression network to reveal the potential regulatory relationships in K. fedtschenkoi. Measurements of leaf titratable acidity, soluble sugar, and starch turnover provided metabolic indicators of the magnitude of CAM under the different light treatments and were used to provide biological context for the expression dataset. Furthermore, CAM-related subnetworks were highlighted to showcase genes relevant to CAM pathway, circadian clock, and stomatal movement. In comparison with white light, monochrome blue/red/far-red light treatments repressed the expression of several CAM-related genes at dusk, along with a major reduction in acid accumulation. Increasing light intensity from an intermediate level (440 ?mol m-2 s-1) of white light to a high light treatment (1,000 ?mol m-2 s-1) increased expression of several genes involved in dark CO2 fixation and malate transport at dawn, along with an increase in organic acid accumulation. CONCLUSIONS:This study provides a useful genomics resource for investigating the molecular mechanism underlying the light regulation of physiology and metabolism in CAM plants. Our results support the hypothesis that both light intensity and light quality can modulate the CAM pathway through regulation of CAM-related genes in K. fedtschenkoi.

Dataset Information

Stomatal Responses to Light, CO₂, and Mesophyll Tissue in Vicia faba and Kalanchoe fedtschenkoi.

Publications

Stomatal Responses to Light, CO<sub>2</sub>, and Mesophyll Tissue in <i>Vicia faba</i> and <i>Kalanchoë fedtschenkoi</i>.

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Dataset Information

Stomatal Responses to Light, CO2, and Mesophyll Tissue in Vicia faba and Kalanchoe fedtschenkoi.

Publications

Stomatal Responses to Light, CO&lt;sub&gt;2&lt;/sub&gt;, and Mesophyll Tissue in &lt;i&gt;Vicia faba&lt;/i&gt; and &lt;i&gt;Kalanchoë fedtschenkoi&lt;/i&gt;.

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OmicsDI is part of the ELIXIR infrastructure

Tweets

Stomatal Responses to Light, CO₂, and Mesophyll Tissue in Vicia faba and Kalanchoe fedtschenkoi.

Stomatal Responses to Light, CO<sub>2</sub>, and Mesophyll Tissue in <i>Vicia faba</i> and <i>Kalanchoë fedtschenkoi</i>.