ClearSeeAlpha: Advanced Optical Clearing for Whole-Plant Imaging.

ABSTRACT: To understand how the body of plants is made, it is essential to observe the morphology, structure and arrangement of constituent cells. However, the opaque nature of the plant body makes it difficult to observe the internal structures directly under a microscope. To overcome this problem, we developed a reagent, ClearSee, that makes plants transparent, allowing direct observation of the inside of a plant body without inflicting damage on it, e.g. through physical cutting. However, because ClearSee is not effective in making some plant species and tissues transparent, in this study, we further improved its composition to prevent oxidation, and have developed ClearSeeAlpha, which can be applied to a broader range of plant species and tissues. Sodium sulfite, one of the reductants, prevented

SUBMITTER: Kurihara D 

PROVIDER: S-EPMC8579160 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature