Sampling the fish gill microbiome: a comparison of tissue biopsies and swabs.

ABSTRACT:

Background

Understanding the influence of methodology on results is an essential consideration in experimental design. In the expanding field of fish microbiology, many best practices and targeted techniques remain to be refined. This study aimed to compare microbial assemblages obtained from Atlantic salmon (Salmo salar) gills by swabbing versus biopsy excision. Results demonstrate the variation introduced by altered sampling strategies and enhance the available knowledge of the fish gill microbiome.

Results

The microbiome was sampled using swabs and biopsies from fish gills, with identical treatment of samples for 16S next generation Illumina sequencing. Results show a clear divergence in microbial communities obtained through the different sampling strategies, with swabbi

SUBMITTER: Clinton M 

PROVIDER: S-EPMC8579561 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature