Membrane protein channels equipped with a cleavable linker for inducing catalysis inside nanocompartments.

ABSTRACT: Precisely timed initiation of reactions and stability of the catalysts are fundamental in catalysis. We introduce here an efficient closing-opening method for nanocompartments that contain sensitive catalysts and so achieve a controlled and extended catalytic activity. We developed a chemistry-oriented approach for modifying a pore-forming membrane protein which allows for a stimuli-responsive pore opening within the membrane of polymeric nanocompartments. We synthesized a diol-containing linker that selectively binds to the pores, blocking them completely. In the presence of an external stimulus (periodate), the linker is cleaved allowing the diffusion of substrate through the pores to the nanocompartment interior where it sets off the in situ enzymatic reaction. Besides the precis

SUBMITTER: Zartner L 

PROVIDER: S-EPMC8580015 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature