CRISPR/Cas9 in zebrafish: An attractive model for FBN1 genetic defects in humans.

ABSTRACT:

Background

Mutations in the fibrillin-1 gene (FBN1) are associated with various heritable connective tissue disorders (HCTD). The most studied HCTD is Marfan syndrome. Ninety percent of Marfan syndrome is caused by mutations in the FBN1 gene. The zebrafish share high genetic similarity to humans, representing an ideal model for genetic research of human diseases. This study aimed to generate and characterize fbn1^+/- mutant zebrafish using the CRISPR/Cas9 gene-editing technology.

Methods

CRISPR/Cas9 was applied to generate an fbn1 frameshift mutation (fbn1^+/- ) in zebrafish. F1 fbn1^+/- heterozygotes were crossed with transgenic fluorescent zebrafish to obtain F2 fbn1^+/- zebrafish. Morphological abnormalities were assessed in F2 fbn1

SUBMITTER: Yin X 

PROVIDER: S-EPMC8580104 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature