Quantitative theory for the diffusive dynamics of liquid condensates.

ABSTRACT: Key processes of biological condensates are diffusion and material exchange with their environment. Experimentally, diffusive dynamics are typically probed via fluorescent labels. However, to date, a physics-based, quantitative framework for the dynamics of labeled condensate components is lacking. Here, we derive the corresponding dynamic equations, building on the physics of phase separation, and quantitatively validate the related framework via experiments. We show that by using our framework, we can precisely determine diffusion coefficients inside liquid condensates via a spatio-temporal analysis of fluorescence recovery after photobleaching (FRAP) experiments. We showcase the accuracy and precision of our approach by considering space- and time-resolved data of protein condensates an

SUBMITTER: Hubatsch L 

PROVIDER: S-EPMC8580480 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature