TOB1 modulates the decidualization of human endometrial stromal cells via the Notch pathway.

ABSTRACT:

Background

Decidualization is critical for embryo implantation and the success of pregnancy; however, the mechanisms underlying this process remain largely unknown.

Materials and methods

In the present study, RNA sequencing was used to detect the expression levels of transducer of ERBB2/1(TOB1) in endometrial samples derived from proliferative and secretory phases. A decidualization model was induced using the combination of estrogen (E2) and progestin (P4) in human endometrial stromal cells (HESCs). The cell counting kit-8 assay was used to detect the viability of HESCs. Related proteins were detected by qPCR and western blot.

Result

The results indicated that TOB1 expression was upregulated in the secretory endometrial samples compared with the corresponding express

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC8581109 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature