Unknown

Dataset Information

0

Mural granulosa cells support to maintain the viability of growing porcine oocytes and its developmental competence after insemination.


ABSTRACT:

Purpose

To enhance the in vitro growth of porcine oocytes, we studied the effect of mural granulosa cells (MGCs) on the viability of oocytes attached to granulosa cells (oocyte-granulosa cell complexes, OGCs) that were obtained from early antral follicles.

Methods and results

When OGCs were cultured with MGCs for 12 days, there were significant improvement (P < 0.05) in the robustness of gap junctional communication between the oocyte and the granulosa cells (82% vs. 59%), the survival rate of oocytes (57% vs. 39%), and the diameter of survived oocytes (118 μm vs. 112 μm). The rate of oocyte release of OGCs cultured with MGCs on the 12th day (1.9%) was significantly lower than that of OGCs cultured without MGCs (26%). Complete meiotic arrest was maintained in the group with MGCs (100%), while partial resumption of spontaneous meiosis was noticed in the absence of MGCs (10-19%). Furthermore, the presence of MGCs increased the oocyte maturation rate after maturation culture in both 12- and 14-day culture groups (P < 0.05, 85-88%) compared to OGCs cultured without MGCs (48-60%). MGCs also significantly improved the blastocyst formation rate (day 7) after ICSI (P < 0.05).

Conclusions

The data of this study thus shows that the presence of MGCs during in vitro oocyte growth plays a crucial role in supporting the developmental competence of growing porcine oocytes attached to the granulosa cells via enhancement of their viability.

SUBMITTER: Yamochi T 

PROVIDER: S-EPMC8581117 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Mural granulosa cells support to maintain the viability of growing porcine oocytes and its developmental competence after insemination.

Yamochi Takayuki T   Hashimoto Shu S   Morimoto Yoshiharu Y  

Journal of assisted reproduction and genetics 20210510 10


<h4>Purpose</h4>To enhance the in vitro growth of porcine oocytes, we studied the effect of mural granulosa cells (MGCs) on the viability of oocytes attached to granulosa cells (oocyte-granulosa cell complexes, OGCs) that were obtained from early antral follicles.<h4>Methods and results</h4>When OGCs were cultured with MGCs for 12 days, there were significant improvement (P < 0.05) in the robustness of gap junctional communication between the oocyte and the granulosa cells (82% vs. 59%), the sur  ...[more]

Similar Datasets

| S-EPMC2845131 | biostudies-literature
| S-EPMC3158269 | biostudies-literature
2019-01-01 | GSE114967 | GEO
2019-11-25 | GSE129994 | GEO
| S-EPMC9490373 | biostudies-literature
| S-EPMC8469918 | biostudies-literature
| S-EPMC5904140 | biostudies-literature
| S-EPMC5320431 | biostudies-literature
| S-EPMC5936615 | biostudies-literature
2013-06-03 | E-GEOD-40916 | biostudies-arrayexpress