Mural granulosa cells support to maintain the viability of growing porcine oocytes and its developmental competence after insemination.

ABSTRACT:

Purpose

To enhance the in vitro growth of porcine oocytes, we studied the effect of mural granulosa cells (MGCs) on the viability of oocytes attached to granulosa cells (oocyte-granulosa cell complexes, OGCs) that were obtained from early antral follicles.

Methods and results

When OGCs were cultured with MGCs for 12 days, there were significant improvement (P < 0.05) in the robustness of gap junctional communication between the oocyte and the granulosa cells (82% vs. 59%), the survival rate of oocytes (57% vs. 39%), and the diameter of survived oocytes (118 μm vs. 112 μm). The rate of oocyte release of OGCs cultured with MGCs on the 12th day (1.9%) was significantly lower than that of OGCs cultured without MGCs (26%). Complete meiotic arrest was maintained in the group with

SUBMITTER: Yamochi T 

PROVIDER: S-EPMC8581117 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature