Spatially resolved quantification of drug metabolism and efficacy in 3D paper-based tumor mimics.

ABSTRACT: Paper-based cultures are an emerging platform for preparing three-dimensional (3D) tissue- and tumor-like structures. The ability to stack individual sheets of cell-containing paper affords a modular means of assembling structures with defined cellular compositions and microenvironments. These layered stacks are easily separated at the end of an experiment, providing spatially resolved populations of live cells for further analysis. Here we describe a workflow in which cell viability, drug penetration, and drug metabolism are quantified in a spatially resolved manner. Specifically, we mapped the distribution of the drug irinotecan and its bioactive metabolite SN38 in a colorectal cancer cell-containing stacked structure with liquid chromatography-mass spectrometry (LC-MS). This paper provi

SUBMITTER: Larson TS 

PROVIDER: S-EPMC8581247 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature