Biological relevance of Granzymes A and K during E. coli sepsis.

ABSTRACT: Aims: Recent in vitro findings suggest that the serine protease Granzyme K (GzmK) may act as a proinflammatory mediator. However, its role in sepsis is unknown. Here we aim to understand the role of GzmK in a mouse model of bacterial sepsis and compare it to the biological relevance of Granzyme A (GzmA). Methods: Sepsis was induced in WT, GzmA^-/- and GzmK^-/- mice by an intraperitoneal injection of 2x10⁸ CFU from E. coli. Mouse survival was monitored during 5 days. Levels of IL-1α, IL-1β, TNFα and IL-6 in plasma were measured and bacterial load in blood, liver and spleen was analyzed. Finally, profile of cellular expression of GzmA and GzmK was analyzed by FACS. Results: GzmA and GzmK are not involved in the control of bact

SUBMITTER: Uranga-Murillo I 

PROVIDER: S-EPMC8581435 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature