Resolving immune cells with patrolling behaviour by magnetic resonance time-lapse single cell tracking.

ABSTRACT:

Background

Immune cells show distinct motion patterns that change upon inflammatory stimuli. Monocytes patrol the vasculature to screen for pathogens, thereby exerting an early task of innate immunity. Here, we aimed to non-invasively analyse single patrolling monocyte behaviour upon inflammatory stimuli.

Methods

We used time-lapse Magnetic Resonance Imaging (MRI) of the murine brain to dynamically track single patrolling monocytes within the circulation distant to the actual site of inflammation in different inflammatory conditions, ranging from a subcutaneous pellet model to severe peritonitis and bacteraemia.

Findings

Single patrolling immune cells with a velocity of <1 µm/s could be detected and followed dynamically using time-lapse MRI. We show, that due to local

SUBMITTER: Masthoff M 

PROVIDER: S-EPMC8581510 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature