Biosensor-Based Optimization of Cutinase Secretion by Corynebacterium glutamicum.

ABSTRACT: The industrial microbe Corynebacterium glutamicum is gaining substantial importance as a platform host for recombinant protein secretion. We recently developed a fluorescence-based (eYFP) C. glutamicum reporter strain for the quantification of Sec-dependent protein secretion by monitoring the secretion-related stress response and now demonstrate its applicability in optimizing the secretion of the heterologous enzyme cutinase from Fusarium solani pisi. To drive secretion, either the poor-performing Pel^SP or the potent NprE^SP Sec signal peptide from Bacillus subtilis was used. To enable easy detection and quantification of the secreted cutinase we implemented the split green fluorescent protein (GFP) assay, which relies on the GFP11-tag fuse

SUBMITTER: Bakkes PJ 

PROVIDER: S-EPMC8581548 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature